Figure 7B shows that the staining intensity of Cyp17a1 was significantly increased in untreated PCOS rats compared with normal control rats (23.17 ± 0.65% vs. 10.96 ± 0.35%, p < 0.05). The increase in staining was significantly reversed (p < 0.05) by treatment with KH + clomiphene (12.81 ± 0.37%), clomiphene alone (12.65 ± 0.38%), KH alone (11.68 ± 0.42%), KH + metformin (11.39 ± 0.37%), and metformin (11.76 ± 0.41%). The gene discussed is CYP17A1; the disease is polycystic ovary syndrome.