To interrogate a causal role of GPR41 and olfr78 in the PVN on programmed hypertension in HFD offspring, gene silencing via microinjection bilaterally into the PVN of lentiviral vectors encoding shRNA targeting GPR41 (Lv-GPR41-shRNA; 1 × 105 IFU) or olfr78 (Lv-olfr78-shRNA; 1 × 105 IFU) was performed at the age of 8 weeks in HFD offspring. This evidence concerns the gene FFAR3 and hypertensive disorder.