CCND1 and malignant colon neoplasm: In HT-29 colon cancer cells, only the extract from 5-ISA-fortified lettuce led to significant 1.2-fold upregulation of pro-apoptotic BAD while anti-apoptotic CCND1 was downregulated by approximately 52% and 40% upon both 5-ISA- and 3,5-diISA-fortified lettuce, respectively, as compared to negative control (Figure 4A,B).