Although the oral bioavailability of EFL1 was low, toxicological studies has reported that the methanol, ethyl acetate, petroleum ether, and aqueous extract of Euphorbia semen cause reduced spontaneous activities, watery stools, wet perioral hair, urinary incontinence, and tics in mice treated with EFL1 (i.g.)once [44,45]. This evidence concerns the gene EFL1 and Tics.