Judging by the findings from the computational analyses in this study, chlorogenic acid established superior interaction and affinity with DPP-IV above Diprotin A. Additionally, in vitro investigation revealed the uncompetitive inhibition of DPP-IV by chlorogenic acid indicating both the in-silico result and in vitro assessment being in tandem with one another, demonstrating the potential of chlorogenic acid as a viable candidate in the management of T2D via the inhibition of DPP-IV. This evidence concerns the gene DPP4 and type 2 diabetes mellitus.