An in vitro experiment using varying concentrations of glucose and insulin showed that high insulin concentration could up-regulate the expression of betatrophin in the case of insulin resistance [50]; the possible mechanism may be attributed to the insulin signaling pathway, that is, insulin binds to insulin receptors on the membrane to activate the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) signaling pathway, as it has been found that the use of PI3K/Akt blocker LY294002 inhibits the stimulating effects of insulin. This evidence concerns the gene ANGPTL8 and Insulin resistance.