The patients (48 ± 17 years, n = 79) who were diagnosed with iron overload were examined for SLC40A1 c.44–24G>C (rs1439816) and c.663T>C (rs2304704) polymorphisms after they were excluded as carriers of genetic hemochromatosis, metabolic diseases, transfusion-dependent pathologies, other possible causes of secondary iron overload, liver disease, and inflammation. This evidence concerns the gene SLC40A1 and Tangier disease.