For the verification set, the AUC of anti-CXCL8 autoantibody was 0.713 (95%CI: 0.624–0.801) and the sensitivity and specificity of anti-CXCL8 autoantibody for ESCC detection were 35.7% and 82.9%, respectively (Figure 4B). This evidence concerns the gene CXCL8 and esophageal squamous cell carcinoma.