Instigated by the recent discovery that SHH, the main ligand and activator of signal transduction through the HH-GLI pathway, can physically bind to AR in androgen-independent PC cell lines [29] and that there are indications for potential physical interaction between SHH and ERα [30], in this study we wanted to determine the expression of AR and SHH in breast cancer tissue samples and correlate it with clinicopathological characteristics, receptors statuses and survival in a cohort of BC patients from Croatia. This evidence concerns the gene ESR1 and pachyonychia congenita.