AFP and malignant colon neoplasm: DMH-colon cancer is primarily dependent on its biotransformation into more reactive intermediates, which takes place via two pathways: glutathione (GSH) conjugation and cytochrome P450 monooxygenases (CYPs)-dependent oxidation (phase I) (phase II) [20]; positive alterations in blood Ca19.9, CEA, and AFP levels were caused by the release of reactive oxygen species (ROS), which harm the colon and create instability in colon cell metabolism.