The ROC analysis (Figure 3) for BMP-9 and HB-EGF, respectively, with estimation of the Youden index, showed for BMP-9 an area under the curve (AUC) of 0.98 and a threshold of 1188 pg/mL (patients correctly classified: 94%), with greater severity in NASH/NAFLD patients with the highest values (Figure 3a). This evidence concerns the gene GDF2 and metabolic dysfunction-associated steatohepatitis.