Treadmill exercise pretreatment blocked the increase in the concentration of IL-1 β (p < 0.001; Figure 4A), IL-6 (p < 0.001; Figure 4B), and TNFα (p < 0.001; Figure 4C) in the prefrontal cortex in 3 × Tg-AD mice. This evidence concerns the gene IL6 and Alzheimer disease.