CFTR and cystic fibrosis: To validate organoid recognition and quantification of organoid swelling in 384-well plates using OrgaQuant, we determined the effect of the CFTR potentiators VX-770 (ivacaftor) and PTI-808 (dirocaftor), which both facilitate opening of the CFTR channel, on FIS in CF nasal organoids with a S1251N CFTR gating mutation.