This analysis revealed that untreated ECHS1 KO cells have increased ‘mucopolysaccharidosis’ (0.72 s.dist), ‘Biotin transport and metabolism’ (0.56 s.dist), and ‘reduction of cytosolic calcium levels’ (0.43 s.dist) compared to untreated CON cells (Figure 3A left column, where red indicates higher expression in untreated ECHS1 KO cells compared to untreated CON cells). This evidence concerns the gene ECHS1 and mucopolysaccharidosis.