Next, the same analysis was repeated by testing the genetics (HLA-DRB1 shared epitope [SE]), biological (ACPA, rheumatoid factor [RF], and the erythrocyte sedimentation rate [ESR]), pre-RA/RA symptoms (tender arthralgia, joint arthralgia, morning stiffness ≥ 60 min), disease scores (DAS28-ESR), and MTX use. This evidence concerns the gene HLA-DRB1 and rheumatoid arthritis.