For case #34 (KRAS-G12D with 26.5% VAF), direct cytofluid pleural effusion (500 μL input) and DNA extracted from this effusion (40 μL input) were used to test the KRAS cartridge, and both resulted in KRAS positive mutation (G12D) with high Cq values of 28.9 and 30.8, respectively. Here, KRAS is linked to Pleural effusion.