We examined the infectivity of pseudotyped SARS-CoV-2, which substitutes the lentiviral VSV-G envelope protein for the SARS-CoV-2 spike protein and contains a gene that can express the GFP protein after infection, when KC was treated to human ACE2/TMPRSSR2-overexpressing HEK293T cells. Here, ACE2 is linked to keratoconus.