We investigated 18F-DPA-714, a TSPO PET radioligand used in many clinical trials, as a potential biomarker evaluating the extent of DIPG tumors in a patient-derived orthotopic xenograft (PDOX) rat model established with HSJD-DIPG-007 primary DIPG human tumor cells. The gene discussed is TSPO; the disease is diffuse intrinsic pontine glioma.