Nonetheless, considering clinical cases on tooth agenesis caused by KDF1 variations are quite limited as yet, additional functional studies, including in vitro cell transfection with the variant plasmids, multi-omics analyses, or RNA-sequencing of the attainable tissue from the patients are required to authentically verify the biological consequences and the pathogenicity of KDF1 variants. The gene discussed is KDF1; the disease is tooth agenesis.