To better understand the transcriptomic features driven by mTOR signaling, we profiled the transcriptome of various mammalian cells with low and high mTOR contents: Tsc1-/- mouse embryonic fibroblasts (MEFs), human breast cancer cell line MCF7, and MDA-MB361 treated with DMSO (mock) or Torin 1, a potent inhibitor of mTOR. The gene discussed is TSC1; the disease is breast cancer.