In this study, we infected RAW264.7 cells with M. bovis C68004 and two non-tuberculous mycobacteria species (NTMs) (Mycobacterium paratuberculosis (MAP) and Mycobacterium smegmatis (M. smegmatis)) separately, and the KLK12 concentration in the cell culture supernatant was measured 24 h after infection. Here, KLK12 is linked to infection.