We evaluated SMAD4P130L expressing cells in MPE samples, which were identified from a long-range, PCR-based, dual deep sequence for targeting the Big 4 Genes KRAS, CDKN2A, TP53, and SMAD4 of pancreatic cancer [14,15] (Supplementary Figure S1 and Table S1). Here, SMAD4 is linked to familial pancreatic carcinoma.