The present study attempts to determine the role of TGFβ and EGF in glioblastoma EGFRvIII-positive cells based on three DK-MG sublines with different EGFRvIII expression status: DK-MGlow, DK-MGhigh, and DK-MGextra-high. The gene discussed is TGFB1; the disease is glioblastoma.