TLR3 and infection: We treated S. prenanti with LPS intraperitoneal injection in this study, and examined the relative mRNA expression of MyD88, TLR2, TLR3, TLR4, TLR18, TLR22-1, TLR22-2 and TLR22-3 genes from S. prenanti in different immune organs (hepatopantras, head kidney, hindgut and spleen) at different infection times, which will help to further clarify the roles of SpTLRs and SpMyD88 genes involved in the antibacterial immunity mechanisms of fish.