In the same experimental group at different ages: the relative expression of Runx-2 mRNA in the 30-day-old leg cartilage tissue in the CHF, BPs-H, and BPs-L groups was higher than that in the 60-day-old group, but there was no significant difference among the groups (p > 0.05). The gene discussed is RUNX2; the disease is congestive heart failure.