RUNX2 and congestive heart failure: The same experimental group between different days and ages: the relative expression levels of Runx-2 mRNA in thoracic vertebra tissue at 30 days of age in CHF-H, CHF-M, and BPs groups were higher than those at 60 days of age, and the CHF-H, CHF-M group, and BPs-H group were significantly higher than 60-day-old at 30-day age (p < 0.05).