Between different experimental groups at the same age: for 30 days of age, the relative expression of OPG mRNA in the thoracic vertebrae of the CHF and BPs groups was higher than that of the NC group, and BPs-H group was significantly higher than the CHF-H group, CHF-L group and BPs-L group (p < 0.05), and the CHF-M group was significantly higher than the BPs-L group (p < 0.05). This evidence concerns the gene TNFRSF11B and congestive heart failure.