However, if the classic sequencing methods do not easily permit the detection of repeated regions, including LINE-1, a current, sensitive method, CHM13h (haploid CHM13h TERT cell line, originally isolated from a hydatidiform mole) permits the study of highly repetitive genomic regions such as centromeres [65,66]; this method applied to human chromosome 8 demonstrated LINE-1 to be present at the centromere of human chromosome 8 [65] and of acrocentric chromosomes [66] (data reported in Supplementary Figure S1); in agreement with FISH mapping of LINE-1 at centromeric position in Homo. This evidence concerns the gene TERT and hydatidiform mole.