We have found that a novel 31 nt tRNAiMet fragment tRiMetF31 generated from miR-34a-guided cleavage [185] can directly target 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), a key proangiogenic factor, and highlighted a crucial role of the miR-34a/tRiMetF31/PFKFB3 axis in CBN-mediated suppression in neuroblastoma biology. Here, PFKFB3 is linked to neuroblastoma.