Analysis of cell proportions in grades II and III IDH1-mutated astrocytoma tumors (n = 32), using single cell and bulk RNAseq data, revealed no significant changes in the proportions of malignant astrocytes, macrophages, oligodendrocytes, and T-cells within a tumor, between grades II and III (Figure 6A). The gene discussed is IDH1; the disease is neoplasm.