In particular, the level of miR-214, a key “melano-miR” involved in melanoma progression and, conveyed in acid EV, was significantly higher than in control EV: miR-214-3p was increased ~2.5-fold in B16 and B16-LU acid-EV and ~4-fold in B16-LI acid-EV compared to control; miR-214-5p, despite less represented than the 3p strand, was upregulated in acid-EV of ~four-fold in all B16, B16-LU, and B16-LI-derived EV (Figure 1D). This evidence concerns the gene BCAM and melanoma.