PMEL and melanoma: We also examined the transcription of melanin synthesis genes TYR, TYRP1, PMEL, MLANA and DCT by RNAseq analysis in M14 human melanoma cells in which we have disrupted TXNRD1 using Crispr/Cas9 (M14TXNRD1+/− cells, Supplementary Figures S3 and S4).