To compare TIMP-1 secretion by CAFs and BC cells, we chose 19TT, immortilized BC-derived CAFs, which, based on cytokine array data, we have previously shown to secret TIMP-1 [15], and AnD5 cells, an MCF-7 subline with a highly homogenous cell population and high responsiveness to CAF-CM [20]. This evidence concerns the gene TIMP1 and breast cancer.