Since p53 acts as an upstream for miR25, the authors again emphasised that the manipulation of associated upstream miR-25 regulatory molecules, such as p53, may prevent ESCC development in high-risk patients, which also corresponds with the research on E-Cadherin; this is summarised above in 3.1, which details how the p53 gene family can up-regulate E-Cadherin expression [60]. This evidence concerns the gene CDH1 and esophageal squamous cell carcinoma.