MAP3K5 and renal carcinoma: We found that mCD40L triggered phosphorylation of the apoptosis-signal regulating kinase 1 (ASK1) which remained phosphorylated even after 6 h post-receptor ligation, particularly in HCT116 cells (Figure 3a); interestingly, unlike our previous observation in bladder and renal cancer cells, the activation of ASK1 was more rapid and exhibited a bi-phasic pattern (evident at 1.5 and 6 h).