Previous FRAP studies performed at room temperature on GFP-tagged mouse Panx1 expressed in a breast cancer cell line (BICR-MIRk) [43] and zebrafish Panx1a expressed in N2a cells [52] revealed diffusion kinetics comparable to those reported here, with recovery of 45–60% at one min after bleaching. Here, PANX1 is linked to breast carcinoma.