Moreover, MAPK3, STAT3, RHOA, MAPK11, HIF1A, FGFR1, GNG5, GNB1, and ARHGEF12 were the potential therapeutic targets for upper ESCC only, whereas PLCG2, EFNA1, CALM1, and RALA were only suitable for lower ESCC. The gene discussed is ARHGEF12; the disease is esophageal squamous cell carcinoma.