In the current study, ITGB1, Col4A1, Col4A2, ITGA6, LAMA4, LAMB1, LAMC1, VWF, ITGA5, THBS1, PDGFB, PGF, RHOC, CTNNB1 (involved in signaling transduction) and UPP1, MGST3, MGST2, AK1, PNP, DAD1, HYAL2, MGLL, COX7A1, and PGM2L1 (involved in cell metabolism) were obtained and considered to be the potential anti-angiogenic targets for both upper and lower ESCC. The gene discussed is HYAL2; the disease is esophageal squamous cell carcinoma.