A US study, one of the largest United Network for Organ Sharing (UNOS) reviews of over 6000 HCC patients within Milan Criteria, found that 5-year survival progressively decreased as AFP increased, with a measurable survival discrimination with an AFP nadir of 15 ng/mL for 5-year survival outcomes (5-year survival: AFP < 15 ng/mL 74%, AFP 16–65 ng/mL 66.1%, AFP > 65 ng/mL 57.4%) [48]. The gene discussed is AFP; the disease is hepatocellular carcinoma.