To assess whether 2-ME induces a cytotoxic effect by inducing apoptosis and/or necrosis, SW1088 glioma cells were treated with 2-ME in a concentration range from 100 pM to 10 μM for 24 h; then, using flow cytometry, the levels of apoptosis and necrosis were assessed using the dye annexin V and propidium iodide (PI). Here, ANXA5 is linked to glioma.