We formerly demonstrated that 2-ME decreases mitochondrial membrane potential in SH-SY5Y neuroblastoma cells [45] and inhibits mitochondrial biogenesis by regulating PGC-1α, COXI, and SIRT3 as a result of the nuclear recruitment of nNOS and NO generation in 143B osteosarcoma cells [26]. The gene discussed is SIRT3; the disease is neuroblastoma.