PCNA and hepatocellular carcinoma: We also confirmed the expression of proliferative marker PCNA as green fluorescence in both the cell lines, and it was significantly decreased to approximately 34% in Hep3B and 57% in Huh7 cells (p < 0.01) in 50 μM alpinumisoflavone-treated cells (Figure 1E,F), thus confirming that alpinumisoflavone reduced the viability and proliferation of HCC cells.