Moreover, in the spheroid formation and 3D spheroid invasion assays, the stemness and invasion of HCC-LM3 cells in the miR-1275 mimic+ EIF5A2 group were enhanced to some extent compared with that in the miR-1275 mimic + EIF5A2-NC group or miR-1275 mimic group (Figure 5L,N,O). This evidence concerns the gene EIF5A2 and hepatocellular carcinoma.