AKT1 and hepatocellular carcinoma: Finally, immunohistochemical staining and WB analyses confirmed that the expression levels of EIF5A2, PI3K and p-Akt declined evidently in Hep-G2 and HCC-LM3 cells treated with genistein miR-1275 mimic, but Akt changed inapparently (Figure S1E,F and Figure 4E).