The LC-MS/MS proteomic analysis of EVs isolated from the serum of a pool of healthy controls and of patients with PTC associated or not with lymph node metastasis (LNM) revealed an increased abundance of proteins of the integrin family (ITGA2, ITGA2B, ITGAV, ITGB1, ITGB2, ITGB3) and of proteins involved in metastasis (TLN1, ITGB2, CAPNS1, SRC) in EVs from PTC patients with LNM. Here, ITGB2 is linked to metastatic malignant neoplasm in the lymph nodes.