BRAF and melanoma: We subsequently analyzed Fhit protein expression in several melanoma cell lines, including SK-Mel28, FO-1, E6LCP, 1259CL93, M14, Hs294T, 1106, and Colo38 having mutations in B-RAF and WM13066, MeWo and CHL-1 having B-RAF WT, as well as normal melanocytes via Western blotting (Figure 1B and data not shown).