RAF1 and melanoma: To this end, melanoma cell lines, Colo38 (B-RAF V600E) and CHL-1 (B-RAF wild-type), were mock- and FHIT-transfected and treated with the inhibitor of Hsp90 known as 17-allylamino-17-dimethoxy geldanamycin (17-AAG) for 24 hrs (Figure 2A,B).