NPPB and cardiac hypertrophy: Furthermore, mice fed the CPD diet did not present with LV hypertrophy as evidenced by unchanged cardiomyocyte area measured by wheat germ agglutinin (WGA) staining, unchanged LV collagen content measured by picrosirius red staining, and similar cardiac mRNA expression of the molecular marker of hypertrophy, Bnp (Figure 2F–H).