Our work offers novel findings for the role of exosomes, as we demonstrate that both the CM and the exosomes statistically significantly downregulated the mRNA expression of the LPS-induced inflammation-related genes (TNF-α, IL1-β, IL-6) in SEMFs, and this downregulation was also confirmed at the protein level of IL-1β, highlighting a possible anti-inflammatory therapeutic strategy for IBD. This evidence concerns the gene IL6 and inflammatory bowel disease.