In addition, TNF-α combined with cycloheximide increased the concentration of sphingosine inside the lysosome, which in turn triggered LMP in rat (HTC), murine (1c1c7), and human HCC (SK-HEP-1) cells, resulting in the release of CTSB and CTSD [79,80]. This evidence concerns the gene CTSB and hepatocellular carcinoma.