Screening of stage-specific transcriptome revealed differential expression of several T1D candidate genes, e.g., TYK2, DLK1, and IFIH1 were strongly upregulated while PRKCQ, BACH2, and GPR183 were downregulated as differentiation progressed towards endocrine lineage (Fig. 1c). This evidence concerns the gene GPR183 and type 1 diabetes mellitus.