Fractionation assay, immunofluorescence, and IHC staining showed that knockdown of FHL2 significantly attenuated the nucleus translocation of β-catenin and further exacerbated the reduced β-catenin nucleus translocation caused by TAB182 knockdown in ESCC cells (Figs. 4C, D and Fig. S2B). The gene discussed is FHL2; the disease is esophageal squamous cell carcinoma.