Prognosis analysis indicated that the hypermethylation of TOP3B in LAML; TOP2B in ACC and CESC; TOP1 in UCS and UVM, TOP1MT in KIRC and THYM was associated with a poor prognosis, while the hypermethylation of TOP2B in LUSC; TOP1 in LGG and KIRC; TOP3A in LGG and KIRC; TOP2A in LGG and PRAD; TOP1MT in DLBC, GBM, HNSC and SKCM was associated with good prognosis (Fig 2C). Here, TOP2B is linked to cervical squamous cell carcinoma.