In this study, the inflammatory cell supernatant produced by THP-1 macrophages under lipopolysaccharide (LPS) stimulation was applied to head and neck tumor cell lines, to establish the inflammatory condition model of tumor cells, to isolate the subpopulation of cells with high LGR5 expression induced by inflammatory factors, and to determine the correlation between LGR5 and EMT by comparing and analyzing the EMT phenotypic transformation ability with parental cells. This evidence concerns the gene LGR5 and head and neck neoplasm.