Notably, most of the IGHV1-69-encoded clones isolated from Omicron BA.1 breakthrough infection utilize IGLV1-40 light-chain gene and have a preferred length of 17 amino-acid HCDR3s and a GYSGYG/D-like motif, which are related to our previous reported R1-32-like antibody lineage (non-ACE2 competitive) that isolated from primary SARS-CoV-2 ancestral strain infection or vaccination (Figure 4(D), Figure S2A, Figure S6B). This evidence concerns the gene ACE2 and infection.