The role of TLR2-mediated neuroinflammation was also evidenced by Chen et al. [56], who demonstrated that the unilateral subcutaneous facial injection of BoNT/A (0.18 U of Lanzhou manufactured BoNT/A into the whisker pad), in a trigeminal neuralgia model induced by CCI of the distal infraorbital nerve in mice, attenuated bilateral trigeminal neuropathic pain behaviors and inhibited the upregulation of microglia in TLR2 expression. This evidence concerns the gene TLR2 and trigeminal nerve disorder.